Two papers on cell collision and capture dynamics in GEDI devices published

We have recently published two papers on the collision and capture dynamics of circulating tumor cells (CTCs) and other rare cells in geometrically enhanced differential immunocapture (GEDI) microdevices.

The first paper, “Cell capture simulations for the optimization of microfluidic rare cell immunocapture devices” was published in Biomedical Microdevices by James Smith, Timothy Lannin, Yusef Syed, S Santana, and Brian Kirby.  We use computational fluid dynamics (CFD), particle advection, and exponential-based cell capture simulations to identify capture-optimized GEDI geometries.  We show that it’s possible to select a geometry which maximizes capture efficiency while rejecting small contaminating cells via infrequent collisions and large contaminating cells via high shear stress; the accompanying figure shows this as capture probability vs. particle diameter in an example GEDI geometry.

Capture probability vs. cell diameter in a GEDI device
The probability of capturing cells in a GEDI device is shown vs. cell diameter; capture probability is a function of collision frequency, the duration of cell-obstacle contact, and the local shear stress. Shaded regions represent standard deviation for N = 30,000 simulated cells.

We have also published a paper in Physical Review E, “Transport and collision dynamics in periodic asymmetric obstacle arrays: rational design of microfluidic rare cell immunocapture devices“, by Jason Gleghorn, James Smith, and Brian Kirby. The paper describes work using CFD and ballistic collision dynamics simulations to better understand how to bring rare cells into contact with an antibody-terminated capture surface in a GEDI device, and describes previously unrecognized collision mode structures and differential size-based collision frequencies that emerge from these arrays.

Copies of both of these papers are available on my website.

Advertisements

Huang gave a talk at BMES Annual Meeting 2013 in Seattle, WA

This past week I traveled to Seattle to give a talk at the Biomedical Engineering Society Annual Meeting. My talk in the Nano to Micro Technologies track was entitled “A Hybrid Dielectrophoresis and Immunocapture System for Enhanced Capture of Circulating Tumor Cells” and described my recent work on enriching prostate cancer cells from blood cells with dielectrophoresis and immunocapture techniques. The session was chaired by Kirby Lab alumnus Ben Hawkins, now an Assistant Professor at San Jose State University.

This was my 4th year in a row attending the BMES Annual Meeting, and it has been amazing to see it grow this quickly… Attendance records are being broken every year! This year I focused on attending the career advice panels and meeting company representatives as I begin a job hunt in the medical device industry.

Lannin Attends BMES Annual Conference; Presents Poster on Machine Learning for Identification of CTCs from Images

I recently traveled to Seattle, WA to attend the Biomedical Engineering Society’s Annual Meeting. There, I presented a poster detailing my advancements on machine learning for the automated identification of circulating tumor cells from microscope images. I discussed the advantages of automated cell identification, for example, its consistent output and order of magnitude increase in speed over manual classifications.

Because there were multiple sessions simultaneously, I couldn’t see everything that I wanted. Nonetheless, I attended many good talks. Below are a few that stood out:

 

High-Throughput Partial Wave Spectroscopic Microscopy for Early Cancer Detection

J. E. CHANDLER, H. SUBRAMANIAN, C. D. MANEVAL, C. A. WHITE, AND V. BACKMAN

Northwestern University, Evanston, IL

 

Effect of Pseudopodial Extensions on Neutrophil Hydrodynamics and Adhesion Binding

A. ROCHELEAU, W. WANG, AND M. KING

Cornell University, Ithaca, NY

 

Computational Field-Portable Microscope for On-Chip Imaging of Confluent Samples

A. GREENBAUM, N. AKBARI, AND A. OZCAN

Electrical Engineering Department, University of California, Los Angeles, CA,

Bioengineering Department, University of California, Los Angeles, CA

 

Low-Voltage Electroosmotic Flow and DNA Shearing Using Ultrathin Nanoporous Silicon Membranes

T. GABORSKI, R. CARTER, J. SNYDER, AND J. MCGRATH

Rochester Institute of Technology, Rochester, NY

University of Rochester, Rochester, NY

 

A micro-Hall Chip for Sensitive Detection of Bacteria

D. ISSADORE, R. WEISSLEDER, AND H. LEE

University of Pennsylvania, Philadelphia, PA

Massachusetts General Hospital – Center for Systems Biology, Boston, MA

 

Nanoscale Roughness and Surface Charge Control Selectin-Mediated Adhesion of Malignant and Non-Malignant Cells Under Flow

M. J. MITCHELL, C. A. CASTELLANOS, AND M. R. KING

Cornell University, Ithaca, NY

 

Vortex Technology for CTC Extraction From Blood Samples

D. E. GO, E. SOLLIER, J. CHE, R. KULKARNI, AND D. DI CARLO

UCLA, Los Angeles, CA, 2Vortex Biosciences, Palo Alto, CA

 

Sheathless, On-Chip Flow Cytometer Enabled by Standing Surface Acoustic Waves (SSAW)

Y. CHEN, L. WANG, AND T. J. HUANG

The Pennsylvania State University, University Park, PA,

Ascent Bio-Nano Technologies, Inc., State College, PA

 

Circulating Tumor Cell Capture Amplification

A. N. HOANG, A. SHAH, T. BARBER, M. PHILLIPS, D. WINOKUR, S. MAHESWARAN, D. A. HABER, S. L. STOTT, AND M. TONER

Harvard Medical School, Boston, MA,

Surgical Services and BioMEMS Resource Center, Massachusetts General Hospital, Charlestown, MA,

Massachusetts General Hospital Cancer Center, Charlestown, MA

 

Many of the posters that I was most interested in were at the same time that I was presenting my own. Nonetheless, I also saw some good posters. Here are some of the impressive ones:

 

The Use of Electrokinetic Phenomena to Characterize Malignant Cells

P. KYLE, L. ANDERS, J. CEMAZAR, C. ROBERTS, E. SCHMELZ, AND R. DAVALOS

Virginia Tech, Blacksburg, VA

 

Dielectric Impedimetric Detection Method for Bacterial Biofilm Cultures under Different Growth Conditions.

J. PAREDES, S. BECERRO, AND S. ARANA

CEIT and Tecnun (University of Navarra), Donostia-San Sebastián, Spain

CIC microGUNE, Arrasate-Mondragon, Spain

 

Determining the Effect of Fluid Shear Stress on the Elastic Properties of Cancer Cells using a Micropipette Aspiration Technique

V. CHIVUKULA, J. T. NAUSEEF, M. HENRY, K. B. CHANDRAN, AND S. C. VIGMOSTAD

The Universit of Iowa, Iowa City, IA