My paper “Enrichment of prostate cancer cells from blood cells with a hybrid dielectrophoresis and immunocapture microfluidic system” was recently published in the journal Biomedical Microdevices. This paper describes my work on characterizing cancer cell enrichment from blood cells using a combination of dielectrophoresis (DEP) and immunocapture techniques. I showed that dielectrophoresis can enhance the capture of prostate cancer cells while reducing the nonspecific adhesion of peripheral blood mononuclear cells to immunocapture surfaces. This work informs the design of future hybrid DEP-immunocapture devices for high-purity rare cell capture.
Huang C, Liu H, Bander NH, Kirby BJ. “Enrichment of prostate cancer cells from blood cells with a hybrid dielectrophoresis and immunocapture microfluidic system,” Biomedical Microdevices, 15(6): 941-8, 2013. DOI
Recently, my paper “Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture” was published in and featured on the cover of ELECTROPHORESIS.
This paper describes how characterization of adhesion of prostate cancer cells to immunocapture surfaces with and without dielectrophoresis (DEP) effects as a function of shear stress was performed in a Hele-Shaw flow cell. Gold interdigitated electrodes were deposited on a glass surface that was functionalized with a prostate-specific monoclonal antibody, J591; these electrodes applied an electric field gradient to attract prostate cancer cells to the immunocapture surface with positive DEP. This work demonstrates that DEP and immunocapture techniques can work synergistically to improve cancer cell capture performance, and it informs the design of future high-purity rare cell capture systems to facilitate genetic and pharmacological evaluation of cancer.
Huang C, S Santana, Liu H, Bander NH, Hawkins BG, Kirby BJ. ”Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture,” Electrophoresis, 34(20): 2970-9, 2013. DOI